Emergence of NDM-1 metallo-β-lactamase in Pseudomonas aeruginosa clinical isolates from Serbia.
نویسندگان
چکیده
This work reports, for the first time, the presence of New Delhi metallo-β-lactamase 1 (NDM-1) in Pseudomonas aeruginosa. Moreover, this is the first report of the NDM-1 presence in the Balkan region. Cosmid gene libraries of carbapenem-nonsusceptible Pseudomonas aeruginosa clinical isolates MMA83 and MMA533 were screened for the presence of metallo-β-lactamases. Accordingly, both MMA83 and MMA533 carried the bla(NDM-1) gene. Pulsed-field gel electrophoresis (PFGE) analysis indicated that strains MMA83 and MMA533 belonged to different clonal groups. Five additional isolates from different patients clonally related to either MMA83 or MMA533 were found to be NDM-1 positive.
منابع مشابه
فراوانی اینتگرون‌های کلاس I و II در ایزوله‌های بالینی سودوموناس آئروژینوزا مولد متالوبتالاکتاماز
Background and Objective: Pseudomonas aeruginosa is an opportunistic nosocomial pathogen. Evidence suggests that the incidence of enzyme-producing strains of Pseudomonas aeruginosa Metalo Beta Lactamases (MBL) is a major problem in the treatment of infections caused by this organism. The aim of this study was to investigate the frequency of class I and II integrons among metalobetalactamase pro...
متن کاملDetection of New Delhi metallo beta lactamase-1 (NDM-1) carbapenemase in Pseudomonas aeruginosa in a single centre in southern India
BACKGROUND & OBJECTIVES New Delhi metallo β-lactamase-1 (NDM-1) producing Pseudomonas aeruginosa isolates are potential threat to human health. This study was conducted to detect the presence of bla(NDM-1) in carbapenem resistant P. aeruginosa in a tertiary care center in southern India. METHODS Sixty one carbapenem resistant clinical isolates of a total of 212 P. aeruginosa isolates cultured...
متن کاملCarriage of blaNDM-1 in Pseudomonas aeruginosa through multiple Inc type plasmids in a tertiary referral hospital of northeast India
Pseudomonas aeruginosa is known to be a predominant opportunistic pathogen and also a frequent cause of nosocomial infection in patients with compromised immune system. Treatment option becomes complicated when this type of organism harbour resistance determinants such as New Delhi metallo-β-lactamase-1 (NDM-1). The genetic vehicles carrying this gene are often responsible for their horizontal ...
متن کاملAssociation Between Metallo-β-lactamases and Integrons with Multi-Drug Resistance in Pseudomonas aeruginosa Isolates
Pseudomonas aeruginosa is among the most important pathogens in the nosocomial infections. A genetic mobile element, the integron, is one of the major agents involved in dissemination of multi-drug resistance among gram negative bacteria. During a descriptive study from October 2009 to August 2010, some 130 P. aeruginosa clinical isolates were collected from different wards of three hospitals...
متن کاملIdentification and Characterization of Metallo-β-Lactamases Producing Pseudomonas aeruginosa Clinical Isolates in University Hospital from Zanjan Province, Iran
Background: Infectious by Pseudomonas aeruginosa has spread worldwide and metallo-beta-lactamases (MBL) are being reported with increasing frequency. The aim of this study was to investigate the antibiotic susceptibility and distribution of blaVIM and blaIMP genes in P. aeruginosa isolates from Zanjan Province of Iran. Methods: A total of 70 P. aeruginosa isolates were identified from patients ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 55 8 شماره
صفحات -
تاریخ انتشار 2011